Antimicrobial Peptide EM86 Efficacy in Infected Mouse Wounds

EM86, a computer-designed antimicrobial peptide, killed multidrug-resistant Pseudomonas aeruginosa SM016 within 60 minutes and showed low toxicity to human skin fibroblasts (IC50 > 300 μg/mL). The study functionalized a sodium alginate/polyvinyl alcohol nanofibrous dressing with 35 ± 18 μg of EM86, which significantly reduced bacterial counts in open-wound infected BALB/c mice after 4 days of treatment. This dataset, authored by Samar Essam Metwally and uploaded in April 2026, documents the in vitro and in vivo results.

Use Cases

• Analyze the relationship between minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values for EM86 against various MDR Gram-negative isolates.
• Model the time-kill kinetics of EM86 against Pseudomonas aeruginosa SM016 using data points collected over 60 minutes.
• Correlate nanofiber dressing properties like mean diameter (238 ± 78 nm) and swelling capacity (510%) with in vivo treatment efficacy.
• Assess the toxicity profile of EM86 by comparing its IC50 value (>300 μg/mL on human skin fibroblasts) to that of colistin.

Strengths

• Includes specific quantitative results such as MIC/MBC values, IC50 > 300 μg/mL, and nanofiber diameter of 238 ± 78 nm.
• Provides in vivo efficacy data from BALB/c mouse model showing significant bacterial count reduction (P < 0.001).

Limitations

• Dataset is extremely small (47.2 KB DOCX file), containing summary results rather than raw, granular experimental data.
• No columnar data or sample records are provided, limiting direct analysis.
• Findings are specific to one bacterial strain (Pseudomonas aeruginosa SM016) and one mouse model, limiting generalizability.

Provenance

Source

figshare, authored by Samar Essam Metwally.

Collection Method

Experimental data from in vitro assays (MIC, MBC, MTT toxicity) and in vivo testing on BALB/c mice.

Time Range

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Freshness

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Geography

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