DF-1 Chicken Fibroblast Multi-Omic Immune Response Data

Frederic Sorgeloos published a dataset on 2026-05-29 containing results from combined RNA-Seq and quantitative proteomic analyses of DF-1 chicken fibroblasts. The data captures gene and protein expression changes following stimulation with type I interferon, poly(I:C), and Infectious Bursal Disease Virus. It includes over 110 consistently upregulated genes and quantification of more than 2,400 proteins.

Use Cases

• Identify conserved antiviral gene signatures based on the core set of over 110 upregulated genes.
• Analyze stimulus-specific transcriptional programs based on responses to interferon, poly(I:C), and IBDV.
• Study transcription-translation coupling based on the correlation between mRNA and protein abundance.
• Investigate post-transcriptional regulation based on proteins with altered expression without corresponding mRNA changes.

Strengths

• Integrates transcriptomic and proteomic data from the same experimental system.
• Quantifies more than 2,400 proteins.
• Identifies a core set of over 110 consistently upregulated genes across three immune stimuli.
• Data is licensed under CC-BY-4.0 for open reuse.

Limitations

• Dataset is small at 45.0 KB, indicating limited scope.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.

Provenance

Source

Frederic Sorgeloos via figshare

Collection Method

RNA-Seq transcriptomics combined with SILAC-based quantitative proteomics on stimulated DF-1 chicken fibroblasts.

Freshness

Last updated 2026-05-29 04:45:30; freshness should be verified.