Horizontal Gene Transfer Events in Five Euplotes Ciliate Genomes

A 2026 study by Ruanlin Wang provides a systematic analysis of horizontal gene transfer (HGT) across five genome-sequenced Euplotes ciliates. The research identifies 342 HGT-acquired genes with annotated functions and includes validation experiments for specific mannan endo-1,4-β-mannosidase genes. The dataset is a 14.8 KB document summarizing the methods, results, and conclusions of this genomic investigation.

Use Cases

• Analyze the list of 342 HGT-acquired genes to study functional enrichment in categories like carbohydrate metabolism and sulfur metabolism.
• Compare genomic features (GC content, intron proportion, CDS length) between HGT-acquired and native genes to infer acquisition timing.
• Examine the five putative endosymbiont-derived glycoside hydrolase genes from E. vannus for symbiosis research.
• Study the seven HGT families putatively transferred into the last common ancestor of all five Euplotes for phylogenetic analysis.
• Use qPCR and RNAi validation data for Ea-ManA and Ea-ManB genes to model gene essentiality in response to feeding.

Strengths

• Analysis is based on five fully genome-sequenced Euplotes species.
• Includes functional annotation from Pfam, GO, and KEGG databases for 342 identified genes.
• Contains experimental validation (qPCR, RNAi) for key gene function claims.

Limitations

• The core data is a summary document (14.8 KB DOCX), not the raw genomic or transcriptomic sequences.
• Sample size is limited to five ciliate species, restricting broader taxonomic conclusions.
• The 'rows' or precise data record count for the gene list is not explicitly provided.

Provenance

Source

Figshare, authored by Ruanlin Wang.

Collection Method

Phylogeny-based HGT detection, transcriptome analysis, and functional annotation via BLAST against Pfam/GO/KEGG, followed by experimental validation.

Time Range

Study published in 2026; temporal coverage of genomic events is evolutionary/historical.

Freshness

Last updated in April 2026, indicating recent analysis.

Geography

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