ant(9)-Ie: Novel Spectinomycin Resistance Gene Characterization in Providencia

A novel resistance gene, designated ant(9)-Ie, was identified in Providencia rettgeri P17, isolated from animal farm wastewater. The recombinant strain harboring ant(9)-Ie showed a 64-fold increase in the MIC of spectinomycin, with a catalytic efficiency of (8.22 ± 1.24) × 10² M⁻¹·s⁻¹. The dataset, authored by Lulu Huang and last updated in June 2026, presents the discovery and characterization results.

Use Cases

• Comparative genomic analysis based on the whole-genome sequencing data mentioned in the description
• Kinetic modeling of enzyme activity based on the reported spectinomycin catalytic efficiency (kcat/Km)
• Phylogenetic analysis of resistance genes based on the amino acid sequence similarity to ANT(9)-Id (58.67%)
• Studying the genetic environment of resistance genes based on the described conserved genetic context in Providencia strains

Strengths

• Includes specific kinetic parameter for the novel enzyme: spectinomycin catalytic efficiency of (8.22 ± 1.24) × 10² M⁻¹·s⁻¹
• Reports a concrete experimental result: a 64-fold increase in Minimum Inhibitory Concentration (MIC) for spectinomycin
• Provides a precise amino acid sequence similarity comparison to ANT(9)-Id (58.67%)

Limitations

• Row count is unknown, which may limit suitability assessment
• Column-level documentation is absent; field semantics must be inferred after download
• The dataset is very small (10.4 KB), indicating limited scope

Provenance

Source

figshare, authored by Lulu Huang

Collection Method

Bacterial isolation from wastewater, MIC determination, molecular cloning, heterologous expression, kinetic analysis, whole-genome sequencing, and comparative genomics.

Freshness

Last updated 2026-06-02 04:28:46; freshness should be verified