Hub Genes in Chronic Hepatitis B Immune-Active Phase from Transcriptomic Analysis

36 differentially expressed genes were identified as specific to the immune-active phase of chronic hepatitis B in the transcriptomic dataset GSE230397. The dataset, created by Fangfang Li and last updated in May 2026, contains hub genes identified via bioinformatics and machine learning approaches, validated with clinical samples and single-cell RNA sequencing data. It is a small, 11.5 KB Excel file shared under a CC-BY-4.0 license.

Use Cases

• Validate identified hub genes (CCR5, IL10RA, KCNA3, SLC24A4) in independent chronic hepatitis B studies based on the description.
• Investigate correlations between gene expression and immune cell infiltration (e.g., M1/M2 macrophages) based on the described analysis.
• Perform structure-based virtual screening for FDA-approved compounds targeting the key hub gene SLC24A4 as mentioned in the methods.
• Benchmark machine learning algorithms for identifying disease-phase-specific biomarkers from transcriptomic data.

Strengths

• Includes validation from two independent single-cell RNA sequencing datasets (GSE182159 and GSE283471) and western blot analysis of clinical samples.
• Identifies four specific hub genes (CCR5, IL10RA, KCNA3, SLC24A4) using an integrated bioinformatics and machine learning pipeline.
• Focuses on a clinically pivotal phase (immune-active) of a major global health burden, chronic hepatitis B.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is very small (11.5 KB), indicating limited scope, likely containing summary results rather than raw expression data.

Provenance

Source

figshare, author Fangfang Li.

Collection Method

Integrated bioinformatics and machine learning analysis of transcriptomic dataset GSE230397, with validation from scRNA-seq and clinical samples.

Time Range

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Freshness

Last updated 2026-05-04 05:31:08; freshness should be verified.

Geography

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