Mouse Duodenal Transcriptome and Microbiome Data for Ochratoxin A-Induced Ferroptosis

A 7-day mouse exposure study from 2026 by Shaokat Ali investigates the intestinal toxicity of Ochratoxin A. The dataset includes transcriptomic analysis revealing 769 differentially expressed genes and 16S rRNA sequencing of cecal contents showing altered microbiota. It was published on figshare under a CC-BY-4.0 license.

Use Cases

• Identify ferroptosis-related gene expression changes based on the 769 differentially expressed genes (DEGs) and 26 overlapping ferroptosis-regulating genes.
• Analyze gut microbiota dysbiosis linked to toxin exposure based on altered Bacteroidota abundance and pathogenic microbiota enrichment.
• Correlate biochemical markers of oxidative stress with histological damage based on duodenal malondialdehyde (MDA), iron, and antioxidant defense measurements.
• Validate key ferroptosis regulator genes (SLC7A11, GSTM1, CP, SLC40A1) in a mouse model based on transcriptomic downregulation data.

Strengths

• Includes multi-modal experimental data: transcriptomics, 16S rRNA sequencing, histology, and biochemical assays.
• Identifies 769 differentially expressed genes (134 upregulated, 635 downregulated) with specific focus on 26 ferroptosis-regulating genes.
• Provides specific biochemical measurements (serum LPS, duodenal MDA, iron, glutathione, SOD, CAT, T-AOC) supporting the ferroptosis hypothesis.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Dataset is very small (8.5 KB), suggesting it may contain summary or processed results rather than raw sequencing reads.

Provenance

Source

figshare

Collection Method

Experimental study exposing mice to Ochratoxin A (2 mg/kg body weight/day) for seven days, followed by transcriptomics, 16S rRNA sequencing, and biochemical/histological analysis.

Time Range

Study duration of 7 days; dataset published in 2026.

Freshness

Last updated 2026-04-13 04:35:16; freshness should be verified.

Geography

Laboratory mouse model; specific geographic location not stated.