Metagenomic Characterization of Infected Diabetic Foot Ulcers from North Africa

25 non-infected US diabetic foot ulcers and 15 infected Libyan DFUs were analyzed via shotgun metagenomic sequencing. The study, authored by Muhanad Abdullah Abdulsamad and last updated in May 2026, documents depth-stratified microbial communities, 80 detected antibiotic resistance genes, and a reconstructed high-quality Pseudomonas aeruginosa genome carrying 220 virulence factors.

Use Cases

• Profiling the microbial resistome and virulome in chronic wounds based on the described detection of antibiotic resistance genes and virulence factors.
• Analyzing depth-dependent community shifts in diabetic foot ulcers based on the described predominance of specific taxa in superficial versus deep wounds.
• Studying the genomic characteristics of opportunistic pathogens like Pseudomonas aeruginosa ST664 based on the reconstructed MAG with detailed gene annotations.
• Comparing pre-infection and infected wound microbiomes based on the two distinct cohorts described from the US and Libya.

Strengths

• Includes a high-quality, reconstructed Pseudomonas aeruginosa MAG with 99.68% completeness and detailed annotation of 220 virulence factors and 60 antibiotic resistance genes.
• Profiles two distinct cohorts: 25 non-infected US DFUs as an ecological reference and 15 infected Libyan DFUs as a primary characterization cohort.
• Documents 80 antibiotic resistance genes across depth groups in clinically non-infected wounds.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset's 462.7 KB size suggests a limited scope, likely containing summary or processed results rather than raw sequence data.

Provenance

Source

Muhanad Abdullah Abdulsamad via figshare.

Collection Method

Shotgun metagenomic sequencing, taxonomic classification, resistome and virulome profiling, and metagenome-assembled genome reconstruction.

Freshness

Last updated 2026-05-20 05:50:43; freshness should be verified.

Geography

North Africa (Libya) and United States.