NGF Administration Effects on GnRH Phenotype in Aging Mouse Hypothalamic NSCs

Jiao Luo's dataset on figshare contains experimental results from a study on nerve growth factor (NGF) administration in aging male SAMP8 mice. The data likely includes measurements of serum hormone levels, immunofluorescence results, and transcriptomic profiling related to GnRH neuron differentiation. The dataset was last updated on 2026-04-24 and is licensed under CC-BY-4.0.

Use Cases

• Analyze the relationship between NGF dose and serum hormone levels (LH, FSH, testosterone) based on time-series measurements mentioned in the description.
• Investigate cellular phenotype changes based on GnRH immunoreactivity and Sox2/GnRH co-localization data from the OVLT region.
• Study in vitro differentiation outcomes of hypothalamic neural stem cells into GnRH-secreting neurons based on transcriptomic and qPCR pathway analysis.

Strengths

• Experimental design includes multiple NGF dosage levels (6.25, 12.5, and 25 μg/kg) and multiple post-injection time points (8 h, 24 h, 96 h, 7 d, 10 d).
• Data combines in vivo endocrine measurements with in vitro cellular differentiation and transcriptomic profiling, suggesting a multi-modal approach.
• Dataset is openly licensed under CC-BY-4.0, facilitating reuse.

Limitations

• Row count and column definitions are unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is small (37.0 KB), indicating limited scope, likely containing summary or key results rather than raw experimental data.

Provenance

Source

figshare, author Jiao Luo.

Collection Method

Data likely originates from controlled laboratory experiments using SAMP8 mouse models, immunofluorescence, hormone assays, and cell culture.

Freshness

Last updated 2026-04-24 05:40:50; freshness should be verified.