Table 5_Physicochemical convergence in antibody CDR3-VH repertoires recognizing phosphorot

Riccardo Galasso's research dataset from 2026 contains physicochemical profiles for 113 shared CDR3-VH clonotypes identified through NGS-guided phage display. The data was generated to analyze convergent binding solutions against phosphorothioate-modified antisense oligonucleotides. It includes descriptors such as charge, aromatic residue patterns, CDR3 length, theoretical pI, and hydrophobicity.

Use Cases

• Identify convergent physicochemical signatures in antibody repertoires based on the described CDR3-VH clonotype properties.
• Prioritize candidate antibody binders for polyanionic targets based on the shared enriched clonotype set.
• Analyze repertoire focusing and diversity reduction during selection rounds based on the described NGS-guided framework.
• Correlate theoretical physicochemical descriptors with functional binding assays for modified oligonucleotides.

Strengths

• Defined set of 113 shared CDR3-VH clonotypes with a specific physicochemical profile.
• Data generation involved two independent phage display biopanning strategies coupled with Illumina MiSeq sequencing.
• Findings are supported by functional validation including ELISA binding and fluorescence microscopy assays.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is small (27.6 KB), indicating limited scope.

Provenance

Source

figshare

Collection Method

Generated via next-generation sequencing-guided phage display and bioinformatic pipeline analysis of VH and VL regions.

Freshness

Last updated 2026-05-29 04:50:48; freshness should be verified.