Antibody CDR3-VH Repertoire Physicochemical Signatures for Oligonucleotide Recognition

Deep sequencing data from a phage display study reveals a shared set of 113 CDR3-VH clonotypes with defined physicochemical properties for binding phosphorothioate-modified oligonucleotides. The dataset, created by Riccardo Galasso and last updated in May 2026, includes results from two independent biopanning strategies and subsequent functional assays. It is a 27.6 KB Excel file containing physicochemical descriptor profiles for enriched antibody sequences.

Use Cases

• Identify convergent physicochemical signatures in antibody repertoires based on the described CDR3-VH properties like positive charge and aromatic residue patterns.
• Prioritize candidate antibody binders for polyanionic targets based on the NGS-guided framework and enrichment analysis described.
• Study repertoire focusing and clonal dominance in phage display selections using the described clonotype counting and CPM normalization data.

Strengths

• Includes a defined set of 113 shared CDR3-VH clonotypes identified from deep sequencing.
• Data is derived from two independent phage display biopanning strategies, suggesting reproducibility.
• Findings are supported by functional assays like ELISA and fluorescence microscopy, as described.

Limitations

• Row count and specific column definitions are unknown, requiring manual inspection after download.
• The dataset's 27.6 KB size indicates a very limited scope, likely containing summary results rather than raw sequencing reads.
• Column-level documentation is absent; field semantics must be inferred from the description.

Provenance

Source

figshare, author Riccardo Galasso.

Collection Method

Generated via next-generation sequencing-guided phage display and a dedicated bioinformatic pipeline for clonotype reconstruction.

Freshness

Last updated 2026-05-29 04:54:24; freshness should be verified.