Temporal Transcriptional Dynamics in a Mouse Model of Cutaneous Leishmaniasis

Longitudinal bulk RNA sequencing data from skin lesions and draining lymph nodes in a BALB/c mouse model infected with Leishmania braziliensis. The dataset includes samples collected at six time points from 2 hours to 77 days post-infection, characterizing differential gene expression, pathway enrichment, and gene co-expression networks. The data was generated by Jessica Lobo-Silva and shared under a CC-BY-4.0 license.

Use Cases

• Identify temporal gene expression patterns based on longitudinal sampling across six time points.
• Analyze conserved inflammatory pathways based on cross-species comparison with human disease.
• Discover potential therapeutic targets based on conserved non-coding RNAs and epigenetic regulators like Mir155 and Kdm6b.
• Investigate stage-specific biological processes based on gene co-expression modules linked to innate responses, tissue damage, and skin barrier remodeling.

Strengths

• Longitudinal design with six distinct time points from 2 hours to 77 days post-infection.
• Cross-species validation showing mouse lesions recapitulated 77% of inflammatory pathways described in human CL.
• Identifies 16 conserved microRNAs and 12 shared epigenetic regulators as potential therapeutic targets.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data is from a specific mouse model (BALB/c ear dermal), which may limit generalizability.

Provenance

Source

Jessica Lobo-Silva via figshare.

Collection Method

Bulk RNA sequencing performed on samples from a BALB/c mouse model infected with Leishmania braziliensis.

Time Range

Samples collected at 2, 6, and 48 hours and at 14, 35, and 77 days post-infection.

Freshness

Last updated 2026-05-12 04:20:10; freshness should be verified.