Bioinformatics Analysis of Bushen Anzhi Recipe for Aging-Related Insomnia

A 2026 study by Haiming Li analyzes gene expression data from 555 samples (342 insomnia, 213 control) to investigate the mechanisms of a Traditional Chinese Medicine formula. The dataset includes results from multi-level network and enrichment analyses of 25 effective components and 16 target genes, validated with rat model experiments. The data is shared under a CC-BY-4.0 license on figshare.

Use Cases

• Validate gene targets like HIF1A, PTGS2, and GSK3B based on the multi-level network analysis described.
• Investigate the association with the Wnt signaling pathway based on the study's conclusion.
• Replicate the screening of 25 effective components and 16 neuroinflammation-related differentially expressed genes.
• Correlate in-silico bioinformatics findings with experimental results from polysomnography and Morris water maze tests mentioned.

Strengths

• Includes data from 555 human samples sourced from the GEO database (GSE98582).
• Analysis is supported by experimental validation using 60 rat models across three groups.
• Results are structured in a 14.3 KB XLSX file, facilitating direct tabular analysis.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for specific modeling tasks.
• The dataset is very small (14.3 KB), indicating limited scope, likely containing summary results rather than raw expression data.

Provenance

Source

Gene Expression Omnibus (GEO) database, Traditional Chinese Medicine Systems Pharmacology and HERB databases.

Collection Method

Bioinformatics analysis of public gene expression data, combined with active component screening from TCM databases and experimental validation in rat models.

Time Range

Study published in 2026; underlying GEO dataset GSE98582 collection date is not specified.

Freshness

Last updated 2026-05 08 05:54:39; freshness should be verified.

Geography

Likely reflects data from the GEO database, which is international; experimental validation was performed on Sprague Dawley rats.