DF-1 Chicken Fibroblast Multi-Omics Data for Innate Immune Response Analysis

Table 6_Combined transcriptomic and proteomic analyses define conserved host signatures during innate immune perturbations in DF-1 chicken fibroblasts.xlsx contains multi-omics data from a study of the avian innate immune response. The dataset, authored by Frederic Sorgeloos and last updated in May 2026, integrates RNA-Seq transcriptomics and SILAC-based quantitative proteomics from DF-1 chicken fibroblasts treated with three immune stimuli. It includes data on over 110 consistently upregulated genes and more than 2,400 quantified proteins.

Use Cases

• Identify conserved antiviral gene signatures based on the core set of over 110 upregulated genes mentioned in the description.
• Analyze stimulus-specific transcriptional programs based on the distinct responses to type I interferon, poly(I:C), and IBDV.
• Study transcription-translation coupling based on the described correlation between mRNA and protein abundance and its attenuation post-stimulation.
• Investigate coordinated cellular metabolic suppression based on the downregulation of mitochondrial and protein synthesis proteins.

Strengths

• Integrates two omics layers, quantifying more than 2,400 proteins alongside transcriptomic data.
• Defines a core set of over 110 consistently upregulated genes across three distinct immune perturbations.
• Uses three specific immune stimuli (chicken type I interferon, poly(I:C), and IBDV) for comparative analysis.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for specific analytical tasks.
• Data is specific to the DF-1 chicken fibroblast cell line, which may limit generalizability to other tissues or species.

Provenance

Source

Frederic Sorgeloos via figshare

Collection Method

RNA-Seq transcriptomics combined with SILAC-based quantitative proteomics on treated DF-1 chicken fibroblasts.

Freshness

Last updated 2026-05-29 04:45:34; freshness should be verified.