Metagenomic Profiles of Infected Diabetic Foot Ulcers from North Africa

First shotgun metagenomic characterization of infected diabetic foot ulcers from North Africa includes data from 15 infected Libyan wounds and 25 non-infected US wounds as a reference. The dataset contains taxonomic classifications, resistome and virulome profiles, and a reconstructed high-quality Pseudomonas aeruginosa genome with 220 virulence factors and 60 antibiotic resistance genes. Author Muhanad Abdullah Abdulsamad published the data on figshare in May 2026.

Use Cases

• Profiling microbial community shifts based on wound depth stratification described in the US reference cohort.
• Identifying antibiotic resistance genes (ARGs) like mecA and the mexAB-oprM efflux system detected in non-infected wounds.
• Analyzing the virulome and resistome of major opportunistic pathogens (e.g., Pseudomonas aeruginosa, Staphylococcus aureus) identified in the Libyan cohort.
• Studying mobile genetic elements based on the 213 elements reported in the reconstructed P. aeruginosa MAG.

Strengths

• Includes a high-quality, reconstructed Pseudomonas aeruginosa genome with 99.68% completeness and 0.89% contamination.
• Profiles 220 virulence factors and 60 confirmed antibiotic resistance genes in a single genome.
• Compares two distinct cohorts: 15 infected Libyan DFUs and 25 non-infected US DFUs for ecological reference.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The 197.7 KB file size suggests a limited scope, likely containing summary results rather than raw sequencing data.

Provenance

Source

figshare, author Muhanad Abdullah Abdulsamad.

Collection Method

Shotgun metagenomic sequencing, taxonomic classification, resistome and virulome profiling, and metagenome-assembled genome (MAG) reconstruction.

Freshness

Last updated 2026-05-20 05:50:44; freshness should be verified.

Geography

Primary cohort from Libya (North Africa); reference cohort from the United States.