Sisal SBP Gene Family Identification and Expression Under Pathogen Stress

Filled with genome-wide identification of 45 AhSBP genes in Agave sisalana, with 18 identified as putative miR156 targets. It integrates comparative transcriptomics, promoter cis-element analysis, and RT-qPCR validation to elucidate the miR156-AhSBP regulatory module under pathogen stress. The study was conducted by Zhiwei Lu and published on figshare in 2026.

Use Cases

• Analyze the expansion mechanisms of the 45 AhSBP genes through phylogenetic and synteny modeling.
• Compare transcriptomic and qPCR expression patterns of miR156 targets like AhSBP9 and AhSBP38 between susceptible and resistant cultivars.
• Investigate promoter cis-elements among the AhSBP gene family to understand regulatory mechanisms.
• Identify potential downstream effector genes, such as AhSBP17 and AhSBP34, for engineering disease resistance.

Strengths

• Identifies a specific set of 45 AhSBP genes with lineage-specific expansion details.
• Integrates multiple analysis methods: genome-wide identification, transcriptomics, promoter analysis, and experimental validation.
• Provides clear regulatory insights, identifying 18 AhSBP genes as putative miR156 targets.

Limitations

• The dataset is very small at 10.7 KB, indicating limited scope and likely summary-level data rather than raw experimental outputs.
• Row and column counts are unknown, limiting assessment of data volume and feature granularity.
• Focus is on a single plant species (Agave sisalana), limiting generalizability to other crops.

Provenance

Source

figshare, authored by Zhiwei Lu.

Collection Method

Genome-wide identification, phylogenetic and synteny modeling, comparative transcriptomics, promoter cis-element analysis, and RT-qPCR validation.

Freshness

Last updated in 2026, indicating projected future data.