Antibody CDR3-VH Repertoire Physicochemical Signatures for Oligonucleotide Recognition

Table 7_Physicochemical convergence in antibody CDR3-VH repertoires recognizing phosphorothioate-modified oligonucleotides backbone.xlsx contains data from a next-generation sequencing-guided phage display study. The dataset, authored by Riccardo Galasso and last updated in May 2026, identifies a shared set of 113 CDR3-VH clonotypes with defined physicochemical profiles associated with binding to modified nucleic acid backbones. It includes results from enrichment analysis and physicochemical descriptor profiling of antibody sequences.

Use Cases

• Training machine learning models to predict antibody-antigen binding based on physicochemical signatures like charge and hydrophobicity mentioned in the description.
• Analyzing repertoire convergence and clonal dominance patterns in antibody selections against specific targets.
• Benchmarking bioinformatic pipelines for CDR3 clonotype reconstruction and enrichment analysis from NGS data.
• Studying the relationship between CDR3 sequence features, such as length distribution and aromatic residue patterns, and binding specificity.

Strengths

• Dataset is derived from deep sequencing on an Illumina MiSeq platform, suggesting a high-resolution source.
• Identifies a specific shared set of 113 CDR3-VH clonotypes with a defined physicochemical profile.
• Results are supported by functional validation assays including ELISA and fluorescence microscopy, as described.

Limitations

• The dataset is very small at 34.0 KB, indicating limited scope.
• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.

Provenance

Source

Riccardo Galasso via figshare.

Collection Method

Data generated via a next-generation sequencing-guided phage display strategy, coupled with targeted amplicon sequencing and a dedicated bioinformatic pipeline.

Freshness

Last updated 2026-05-29 04:50:47; freshness should be verified.