Table 8_Functional and genomic characterization of LCN2-deficient PC-3 cells reveals insig

A 13.7 MB Excel dataset by Kiara Gäberlein, last updated April 20, 2026, under a CC-BY-4.0 license. The data presents a functional and genomic comparison between parental PC-3 prostate cancer cells and a CRISPR/Cas9-generated LCN2-deficient clone. Analysis includes STR profiling, next-generation sequencing, RT-qPCR validation, and spectral karyotyping to investigate LCN2's role in cancer progression.

Use Cases

• Identify differentially expressed genes based on next-generation sequencing and RT-qPCR validation data.
• Analyze chromosomal instability based on spectral karyotyping (SKY) and micronuclei data.
• Investigate gene function in cytoskeletal organization and stress response based on described F-actin dynamics and ER stress pathways.
• Validate cell line authenticity for experimental models based on short tandem repeat (STR) analysis.

Strengths

• Data is derived from a defined experimental system using CRISPR/Cas9 knockout and validated STR profiling.
• Analysis integrates multiple techniques: sequencing, karyotyping, and functional assays as described.
• Dataset is openly shared under a permissive CC-BY-4.0 license.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data is specific to a single PC-3 cell line model, which may limit generalizability.

Provenance

Source

figshare

Collection Method

Genetic analysis of cell lines using CRISPR/Cas9, STR profiling, next-generation sequencing, and RT-qPCR.

Time Range

Experimental timeframe not specified.

Freshness

Last updated 2026-04-20 09:07:39; freshness should be verified.

Geography

Geographic origin of the research not specified.