Canine Hemangiosarcoma MicroRNA Expression Profiles from FFPE Tissues

24 dogs with histologically confirmed visceral hemangiosarcoma (18 splenic, six cardiac) and 12 non-neoplastic controls were analyzed via small RNA sequencing and RT-qPCR. The dataset, authored by Laura Machado Ribas and last updated in March 2026, contains differential expression results for 67 miRNAs in splenic and 71 in cardiac HSA, with 18 shared between types. Thirteen miRNAs were validated as differentially expressed, and pathway analysis implicated oncogenic signaling pathways.

Use Cases

• Identify potential diagnostic miRNA biomarkers for canine visceral hemangiosarcoma based on differential expression profiles.
• Validate small RNA sequencing findings using RT-qPCR results for candidate miRNAs.
• Perform pathway enrichment analysis based on validated miRNAs to explore oncogenic signaling mechanisms.
• Compare miRNA expression signatures between splenic and cardiac tumor subtypes within the dataset.

Strengths

• Includes data from 36 canine subjects (24 cases, 12 controls) across two tissue types.
• Validation step confirms differential expression for 13 miRNAs, showing strong concordance with sequencing.
• Pathway enrichment analysis links findings to specific oncogenic pathways like PI3K-Akt and MAPK.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Data may reflect bias inherent to the specific study's sample collection and methodology.

Provenance

Source

figshare, author Laura Machado Ribas.

Collection Method

Analysis of formalin-fixed, paraffin-embedded (FFPE) tissues using small RNA sequencing and quantitative PCR.

Time Range

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Freshness

Last updated 2026-03-17 14:39:55; freshness should be verified.

Geography

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