Temporal Transcriptional Dynamics in a Mouse Model of Cutaneous Leishmaniasis

A longitudinal transcriptomic analysis of skin lesions and draining lymph nodes in BALB/c mice infected with Leishmania braziliensis, performed at 2, 6, and 48 hours and at 14, 35, and 77 days post-infection. The dataset characterizes differential gene expression, pathway enrichment, and gene co-expression networks, with ulcerated mouse lesions recapitulating 77% of inflammatory pathways described in human CL. Jessica Lobo-Silva authored this dataset, last updated on 2026-05-12.

Use Cases

• Identify stage-specific gene modules for early innate responses, tissue damage, and skin barrier remodeling based on the described co-expression analysis.
• Validate potential therapeutic targets by analyzing conserved microRNAs and epigenetic regulators like Mir155 and Kdm6b mentioned in the cross-species comparison.
• Study macrophage polarization and nitric oxide metabolism dynamics using the described upregulation of genes such as Il12a, Il12b, Arg1, and Nos2.
• Investigate the persistence of inflammatory pathways during clinical healing by comparing gene expression profiles at Day 35 and Day 77 post-infection.

Strengths

• Provides a longitudinal framework with 6 distinct time points from 2 hours to 77 days post-infection.
• Includes cross-species validation, with mouse lesions recapitulating 77% of inflammatory pathways found in human cutaneous leishmaniasis.
• Identifies 16 conserved microRNAs and 12 shared epigenetic regulators as potential therapeutic targets.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is small (21.7 KB), indicating limited scope or a summary-level view rather than raw sequencing data.

Provenance

Source

figshare

Collection Method

Bulk RNA sequencing of skin lesions and draining lymph nodes from a BALB/c mouse ear dermal model infected with Leishmania braziliensis.

Time Range

Data collected from 2 hours to 77 days post-infection.

Freshness

Last updated 2026-05-12 04:20:11; freshness should be verified.