TIL Gene Family Analysis in Five Brassicaceae Species and BrTIL1 Cold Tolerance Study

A 2026 study by Zhengnan Xu provides a systematic analysis of the Temperature-induced lipocalin (TIL) gene family across five Brassicaceae species, identifying 23 TIL genes. The research includes functional validation of the Brassica rapa TIL1 (BrTIL1) gene, showing its role in enhancing cold tolerance through overexpression experiments in Arabidopsis thaliana. The dataset, shared on figshare, contains the comparative analysis results in an Excel file.

Use Cases

• Comparative genomics of the TIL gene family based on identified gene structures and evolutionary relationships.
• Studying gene expression under abiotic stress based on the upregulation of BrTIL1 under low-temperature conditions.
• Identifying protein-protein interactions relevant to cold tolerance based on the six BrTIL1-interacting proteins found via yeast two-hybrid screening.
• Analyzing conserved regulatory elements based on the cis-acting elements mentioned in the study.

Strengths

• Includes a systematic analysis of 23 TIL genes across five related plant species.
• Provides functional validation data, including survival rates and enzyme activity levels (POD, CAT, SOD) from overexpression experiments.
• Dataset is openly licensed under CC-BY-4.0.

Limitations

• Row count and column-level documentation are absent; field semantics must be inferred after download.
• The dataset is small (11.2 KB), indicating limited scope, likely containing summary analysis tables rather than raw experimental data.

Provenance

Source

Zhengnan Xu via figshare.

Collection Method

Comparative genomic analysis and laboratory-based functional study (gene expression, yeast two-hybrid, plant assays).

Freshness

Last updated 2026-03-18 07:43:23.