Temporal Transcriptional Dynamics in a Mouse Model of Cutaneous Leishmaniasis

A longitudinal transcriptomic analysis of skin lesions and draining lymph nodes from BALB/c mice infected with Leishmania braziliensis, sampled at 2, 6, and 48 hours and at 14, 35, and 77 days post-infection. The dataset includes differential gene expression, pathway enrichment, and gene co-expression network analysis, identifying conserved inflammatory pathways and potential therapeutic targets. It was authored by Jessica Lobo-Silva and shared under a CC-BY-4.0 license.

Use Cases

• Identify temporal gene expression patterns based on longitudinal sampling at six time points.
• Analyze conserved inflammatory pathways based on the 77% recapitulation of human CL pathways in ulcerated mouse lesions.
• Investigate host-directed therapeutic targets based on conserved microRNAs and epigenetic regulators like Mir155 and Kdm6b.
• Study stage-specific biological processes based on gene modules associated with innate responses, tissue damage, and skin barrier remodeling.

Strengths

• Longitudinal design with six distinct time points from 2 hours to 77 days post-infection.
• Cross-species validation showing ulcerated mouse lesions recapitulated 77% of inflammatory pathways from human CL.
• Identifies 16 conserved microRNAs and 12 shared epigenetic regulators as potential therapeutic targets.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The 46.5 KB file size suggests a limited scope, likely containing summary results rather than raw sequencing data.

Provenance

Source

figshare

Collection Method

Bulk RNA sequencing of skin lesions and draining lymph nodes from a BALB/c mouse ear dermal model infected with Leishmania braziliensis.

Time Range

Samples collected from 2 hours to 77 days post-infection.

Freshness

Last updated 2026-05-12 04:20:13; freshness should be verified.