Tadpole Doctor alignment files

UK-based citizen science project 'Tadpole Doctor' collected tadpole and sediment samples from the UK, Alaska, Florida, and the Czech Republic to investigate Severe Perkinsea Infections (SPI). The dataset contains 3.4 MB of FASTA files with long-read SSU-ITS rDNA sequences, revealing molecular diversity within the Perkinsea clade NAG01. It was authored by Camille Poirier and last updated on June 1, 2026.

Use Cases

• Phylogenetic analysis of the Perkinsea clade NAG01 based on SSU-ITS rDNA sequences.
• Detection of novel parasitic lineages in amphibians based on sequences associated with tadpole livers.
• Comparison of pathogenic Perkinsea Clade (PPC) prevalence across different geographic regions mentioned in the study.
• Assessment of citizen science sample collection for monitoring amphibian disease distribution.

Strengths

• Includes samples from multiple geographic regions: UK, Alaska, Florida, and the Czech Republic.
• Uses a semi-targeted long-read SSU-ITS rDNA sequencing approach, which the description suggests reveals unprecedented molecular diversity.
• Data is openly available under a CC-BY-4.0 license.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Data may reflect geographic bias inherent to the citizen science collection method.

Provenance

Source

figshare, author Camille Poirier.

Collection Method

Samples collected via citizen science; DNA sequenced using a semi-targeted long-read SSU-ITS rDNA approach.

Freshness

Last updated 2026-06-01 11:34:54; freshness should be verified.

Geography

United Kingdom, Alaska (USA), Florida (USA), Czech Republic.