TagSeq Gene Expression for Four Plant Species at the Santa Rita Experimental Range

TagSeq analysis scripts and assembled transcriptomes for four vascular plant species from the Santa Rita Experimental Range in Arizona. The dataset includes transcriptomes for native and introduced species, with raw sequencing reads available under NCBI BioProject PRJNA599443. Hannah E. Marx from the University of Michigan authored the associated pilot study manuscript.

Use Cases

• Benchmarking TagSeq analysis pipelines based on the provided scripts and transcriptome assemblies.
• Studying gene expression patterns in non-model plant species based on the assembled transcriptomes.
• Comparing transcriptomic profiles between native and introduced plant species from the same arid environment.
• Developing methods for RNA-seq analysis in species lacking reference genomes, based on the described assembly process.

Strengths

• Includes data for four distinct plant species, both native and introduced, providing a basis for comparison.
• Raw sequencing reads are publicly available under a specific NCBI BioProject identifier (PRJNA599443).
• Detailed methodological description for RNA extraction, library preparation, and sequencing is provided.

Limitations

• Row count and dataset scale are unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Last update date is unknown; freshness unverified.

Provenance

Source

University of Michigan, associated with a pilot study manuscript.

Collection Method

RNA was extracted from plant tissue collected in 2017, with libraries sequenced on an Illumina NextSeq500 platform.

Time Range

Samples were collected during the 2017 growing season.

Geography

Santa Rita Experimental Range (SRER) Long Term Research and Core NEON site in Arizona, USA.