BosGru_T2T1.0: A Telomere-to-Telomere Reference Genome for the Pamir Yak

BosGru_T2T1.0 is a gap-free, telomere-to-telomere genome assembly for the domestic Pamir yak (Bos grunniens domesticus). The assembly spans 2.66 Gb across 30 chromosomes, contains annotations for 28,596 protein-coding genes, and was generated using PacBio HiFi, Oxford Nanopore ultralong, and Hi-C sequencing data. It serves as a high-quality reference for comparative genomics, variant discovery, and multi-omics studies of yak adaptation and diversity.

Use Cases

• Comparative genomic and population structure analysis based on the high-quality chromosome-scale assembly.
• Variant calling and SNP discovery leveraging the assembly's higher transition/transversion ratio performance.
• Characterizing epigenetic modifications and chromatin architecture using the refined CENP-A, H3K27ac, and H3K27me3 data.
• Refining gene annotations and expression studies with the increased set of 28,596 protein-coding genes.

Strengths

• Assembly is gap-free (telomere-to-telomere) with a contig N50 of 96.04 Mb and high completeness (96.9% BUSCO score).
• Contains annotations for 28,596 protein-coding genes, a significant increase over previous yak genome assemblies.
• Demonstrated superior performance in variant calling, identifying more SNPs with a higher transition/transversion ratio.

Limitations

• Column names and specific row counts for associated data files (e.g., GFF3, XLS) are not provided in the metadata.
• The dataset's primary focus is the Pamir yak breed; applicability to all yak genetic diversity may be limited.
• Metadata from both sources is identical, offering no additional descriptive details on supplementary files.

Provenance

Source

Siyuan Kong

Collection Method

Generated by integrating PacBio HiFi, Oxford Nanopore ultralong, and Hi-C sequencing data.

Freshness

Last updated 2026-03-18.

Geography

Pamir Plateau; relevant to high-altitude regions like the Qinghai-Tibetan Plateau.