ISWI-Nucleosome Crosslinked Complex: Structure in ADP-Bound State

A structural biology dataset from Tsinghua University, authored by Lijuan Yan, detailing the crosslinked complex of the ISWI chromatin remodeler bound to a nucleosome in the ADP state. The data reveals local DNA distortion and translocation mechanisms induced by ISW1, which appear identical to those of the Snf2 remodeler, suggesting a common mechanism. The histone core remains largely unperturbed, and crosslinking did not inhibit the activity of yeast ISW1 or its human homolog.

Use Cases

• Modeling DNA translocation mechanisms based on the described local DNA distortion.
• Comparing chromatin remodeler activity based on the similarity between ISW1 and Snf2 mechanisms.
• Studying protein domain rearrangement during nucleosome binding based on the described catalytic domain activation.
• Investigating histone core stability during remodeling based on the finding that the core remains largely unperturbed.

Strengths

• Data is derived from a high-resolution structure, as mentioned in the description.
• Findings include a direct comparison to the Snf2 chromatin remodeler, suggesting a common mechanism.
• Experimental results include activity tests with crosslinking on both yeast ISW1 and its human homolog.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Last update date is unknown; freshness unverified.

Provenance

Source

paperswithcode, Tsinghua University

Collection Method

Likely contains structural data from experimental methods like cryo-EM or X-ray crystallography, as inferred from the description of a high-resolution structure.