CLDN6 Gene Function in Germ Cell Tumor Cell Lines

Experimental data characterizes the functional and molecular role of the tight junction protein CLDN6 in germ cell tumors. The dataset includes results from CRISPR/Cas9 gene editing, proliferation assays, transcriptomic RNA-sequencing, and proteomic mass spectrometry analyses. It was created by Daniel Nettersheim and published on figshare in April 2026.

Use Cases

• Analyze transcriptome-wide RNA-sequencing data to identify gene expression changes in CLDN6-deficient cell lines.
• Correlate proteome-wide mass spectrometry results with cell adhesion and proliferation assay outcomes.
• Train models to predict cellular aggregation capacity from flow cytometry protein level validation data.
• Compare functional impacts of CLDN6 loss across multiple GCT cell lines (TCam-2, 2102EP, NCCIT, JAR) using PCR and qRT-PCR validation data.

Strengths

• Data generated from four distinct germ cell tumor cell lines for comparative analysis.
• Includes multimodal validation on genome, mRNA, and protein levels.
• Results from both 2D and 3D cell culture model experiments.

Limitations

• Dataset scale is limited to 19.6 MB, suggesting a focused experimental study rather than a large-scale repository.
• Findings are specific to in vitro cell line models, limiting direct translation to patient tumors.
• Sample size (number of biological replicates) for assays is not specified.

Provenance

Source

Daniel Nettersheim.

Collection Method

Experimental data from CRISPR/Cas9 gene editing, cell-based functional assays, RNA-sequencing, and mass spectrometry.

Time Range

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Freshness

Last updated in April 2026.

Geography

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