Human Gut Microbiome Metabolism of 12 Bioactive Coumarins

12 structurally diverse bioactive coumarins were screened for metabolism by the human gut microbiome using semitargeted LC-MS/MS metabolomics and ex vivo fecal cultures. The dataset, authored by Gabrielle Mingolelli and last updated in April 2026, demonstrates a single metabolic pathway reducing the α,β-unsaturated lactone in multiple coumarins, impacting their bioactivity.

Use Cases

• Identify gut microbial species capable of metabolizing coumarins based on the monoculture screen of 11 species.
• Study the impact of microbial metabolism on drug cytotoxicity based on the diminished effect of methoxsalen metabolites on melanoma cells.
• Explore the scope of the α,β-unsaturated lactone reduction pathway across different coumarin structural classes mentioned in the description.

Strengths

• Screens 12 structurally diverse bioactive coumarins for microbial metabolism.
• Includes data from a monoculture screen identifying 11 metabolizing species.
• Demonstrates a direct functional link via N-ethylmaleimide reductase from Escherichia coli.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data may reflect bias inherent to the specific ex vivo fecal culture methodology.

Provenance

Source

figshare, author Gabrielle Mingolelli

Collection Method

Semitargeted LC-MS/MS metabolomics and ex vivo cultures of human feces.

Freshness

Last updated 2026-04-28 10:14:49; freshness should be verified.