Rat and Hamster Liver Proteome with Global Profiling and Targeted CYP Quantification

A global proteomic profiling of rat and hamster liver S9 fractions under basal conditions and following induction with Aroclor 1254 or phenobarbital/β-naphthoflavone. More than 8500 proteins were quantified across conditions, with complementary targeted analysis providing absolute quantification of 15 cytochrome P450 enzymes. The dataset, created by Jasjot Singh and last updated in June 2026, serves as a reference for DMPK, toxicology, and nitrosamine activation studies.

Use Cases

• Modeling xenobiotic pathway remodeling based on global proteomic changes after chemical induction.
• Comparing species-specific cytochrome P450 induction responses between rats and hamsters.
• Calibrating in vitro systems for drug metabolism studies using absolute quantification data for 15 CYP enzymes.
• Investigating the relationship between mitochondrial electron transport downshift and proteostasis network activation under toxicant exposure.

Strengths

• Quantifies more than 8500 proteins across experimental conditions.
• Includes absolute quantification data for 15 specific cytochrome P450 enzymes using stable isotope-labeled standards.
• Compares proteomic responses across two species (rat and hamster) and multiple chemical inducers.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is very small (10.5 KB), suggesting it contains summary or processed results rather than raw spectral data.

Provenance

Source

figshare

Collection Method

Global proteomic profiling and targeted mass spectrometry analysis of liver S9 fractions.

Freshness

Last updated 2026-06-02 09:32:15