Rat and Hamster Liver Proteome with Global Profiling and Targeted CYP Quantification

More than 8500 proteins were quantified in rat and hamster liver S9 fractions under basal and induced conditions. The dataset integrates global proteomic analysis with absolute quantification of 15 cytochrome P450 enzymes involved in drug and nitrosamine metabolism. Jasjot Singh published the data on figshare in 2026.

Use Cases

• Modeling xenobiotic pathway remodeling based on proteomic changes following Aroclor 1254 or PBNF induction.
• Comparing species-specific cytochrome P450 induction responses, such as CYP3A in rats and CYP2F/CYP2G in hamsters.
• Calibrating in vitro systems for CYP-mediated oxidative capacity based on absolute quantification data for 15 P450 enzymes.
• Studying proteostasis network activation, including heat-shock chaperones, in response to chemical stressors.

Strengths

• Quantifies over 8500 proteins across experimental conditions.
• Includes absolute quantification for 15 specific cytochrome P450 enzymes using stable isotope-labeled standards.
• Compares two rodent species (rat and hamster) under multiple induction regimes.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data may reflect experimental bias inherent to the specific induction protocols and S9 fraction preparation.

Provenance

Source

figshare

Collection Method

Global proteomic profiling and targeted mass spectrometry analysis of liver S9 fractions.

Freshness

Last updated 2026-06-02 09:32:12; freshness should be verified.