Rat and Hamster Liver Proteome with CYP Quantification Under Chemical Induction

A global proteomic profiling of rat and hamster liver S9 fractions under basal conditions and following induction with Aroclor 1254 or phenobarbital/β-naphthoflavone. More than 8500 proteins were quantified across conditions, with complementary targeted analysis providing absolute quantification of 15 cytochrome P450 enzymes. The dataset, authored by Jasjot Singh and last updated in June 2026, serves as a reference for DMPK, toxicology, and nitrosamine activation studies.

Use Cases

• Modeling species-specific cytochrome P450 induction responses based on global proteomic changes.
• Studying xenobiotic pathway remodeling in liver tissue following chemical induction.
• Calibrating in vitro systems for drug metabolism studies using absolute quantification of key CYP enzymes.
• Investigating proteostasis network activation, such as heat-shock chaperones, in response to toxicant exposure.

Strengths

• Quantifies more than 8500 proteins across experimental conditions.
• Includes absolute quantification of 15 specific cytochrome P450 enzymes using stable isotope-labeled standards.
• Compares two rodent species (rat and hamster) under multiple induction regimes.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is very small (11.8 KB), indicating limited scope or highly summarized data.

Provenance

Source

figshare

Collection Method

Global proteomic profiling and targeted mass spectrometry with stable isotope-labeled standards.

Freshness

Last updated 2026-06-02 09:32:17; freshness should be verified.