Rat and Hamster Liver Proteome: Global Profiling and Targeted CYP Quantification

Jasjot Singh's dataset provides global proteomic profiling of rat and hamster liver S9 fractions under basal and induced conditions, quantifying over 8500 proteins. The study integrates this global analysis with absolute quantification of 15 cytochrome P450 enzymes using stable isotope-labeled standards. The data was uploaded to figshare on 2026-06-02 and serves as a reference for DMPK, toxicology, and nitrosamine activation studies.

Use Cases

• Modeling xenobiotic pathway remodeling based on proteomic changes following inducer exposure.
• Comparing species-specific cytochrome P450 induction responses between rats and hamsters.
• Calibrating in vitro systems for drug metabolism studies using absolute quantification of 15 CYP enzymes.
• Investigating the relationship between mitochondrial electron transport and proteostasis network activation under chemical stress.

Strengths

• Quantifies over 8500 proteins across experimental conditions.
• Includes absolute quantification for 15 specific cytochrome P450 enzymes.
• Compares data from two species (rat and hamster) under multiple induction regimes.
• Data is structured in an XLSX file, likely facilitating direct analysis.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for certain analyses.
• The 1.9 MB file size suggests a relatively small-scale dataset.

Provenance

Source

figshare, author Jasjot Singh.

Collection Method

Global proteomic profiling and targeted mass spectrometry with stable isotope-labeled standards.

Freshness

Last updated 2026-06-02 09:32:11; freshness should be verified.