E. coli Lysogenization by Phi24B Phage with O Antigen Loss

Research from the Russian Academy of Sciences investigates the lysogenization of non-O157 Escherichia coli strains by the Shiga-toxin-converting bacteriophage phi24B. The study demonstrates that the O antigen effectively limits lysogenization, with lysogens forming from spontaneous rough mutants. These mutants show increased sensitivity to other bacteriophages and serum bactericidal activity compared to parental strains.

Use Cases

• Modeling phage-host range determinants based on O antigen presence.
• Studying bacterial fitness trade-offs associated with lysogenization.
• Investigating the emergence of new pathogenic STEC strains through horizontal gene transfer.
• Analyzing the impact of spontaneous mutations on bacterial susceptibility to environmental threats.

Strengths

• Focuses on a paradigmatic model (phi24B phage) for stx-converting phages.
• Identifies a specific bacterial surface structure (O antigen) as a key factor limiting lysogenization.
• Compares phenotypic outcomes (sensitivity to phages and serum) between mutant and parental strains.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Last update date is unknown; freshness unverified.

Provenance

Source

Russian Academy of Sciences

Collection Method

Likely contains experimental results from microbiological assays.