Drosophila Brat Protein RNA-Binding Mutant Phenotype Data

Data from a 2026 study demonstrating the essential role of RNA-binding for the Drosophila Brat protein's function. The dataset includes results from CRISPR-engineered mutant alleles, complementation tests, lethal phase analysis, and RT-qPCR on target mRNAs. It was authored by Robert Connacher and shared under a CC-BY-4.0 license.

Use Cases

• Validate the functional importance of specific protein residues for RNA binding based on structure-guided mutagenesis.
• Analyze developmental phenotypes and lethal stages associated with loss-of-function mutations in the brat gene.
• Correlate loss of RNA-binding activity with dysregulation of target mRNA expression levels based on RT-qPCR data.
• Study neural stem cell differentiation defects based on neuroblast marker expression patterns in mutant larvae.

Strengths

• Includes data from three independent RNA-binding defective mutant alleles generated by CRISPR-mediated scarless gene editing.
• Contains multiple experimental validations: complementation tests, lethal phase analysis, and RT-qPCR on target mRNAs.
• Shared under a permissive CC-BY-4.0 license for reuse.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data may reflect the specific experimental focus and model system inherent to the source study.

Provenance

Source

figshare

Collection Method

Experimental data from structure-guided mutagenesis, genome engineering, and phenotypic analysis in Drosophila.

Freshness

Last updated 2026-05-28 10:20:05.