LepI C52A: X-Ray Crystal Structures with SAM and Leporin C

X-ray crystal structures of the SAM-dependent pericyclase enzyme LepI, solved in complex with S-adenosylmethionine (SAM) and the product leporin C. The dataset includes structures with a substrate analogue and a retro-Claisen reaction transition-state analogue, revealing the structural basis for multiple catalytic reactions. The data was produced by Yujuan Cai of the Chinese Academy of Sciences.

Use Cases

• Analyzing enzyme active site architecture based on structural coordinates of LepI with bound ligands.
• Studying the evolution of catalytic function based on mutational analysis of residues H133 and R295.
• Modeling reaction mechanisms like intramolecular Diels-Alder cyclizations based on transition-state analogue structures.
• Investigating the role of SAM cofactors in non-methyltransferase catalysis as proposed in the description.

Strengths

• Structures solved for multiple states: SAM-bound, product-bound, and with a transition-state analogue.
• Analysis includes mutational studies on specific active-site residues H133 and R295.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Last update date is unknown; freshness unverified.

Provenance

Source

Yujuan Cai, Chinese Academy of Sciences

Collection Method

X-ray crystallography