Time-Resolved Proteomic Analysis in Zebrafish Using Bioorthogonal Noncanonical Amino Acid

Bioorthogonal noncanonical amino acid tagging (BONCAT) proteomic analysis in larval zebrafish, with labeling durations as short as 12 hours. Sophie E. Miller published the dataset on figshare, last updated on 2026-04-14. The data was used to investigate proteomic changes in response to heat shock, revealing upregulated proteins related to stress responses, lipid metabolism, and neural regulation.

Use Cases

• Analyzing time-resolved protein synthesis based on BONCAT labeling durations mentioned in the description
• Investigating heat shock response mechanisms based on identified upregulated proteins
• Comparing targeted proteomic sensitivity to global proteomics based on the described proof of concept
• Studying stress-induced changes in lipid metabolism and neural regulation based on the findings

Strengths

• Data demonstrates significant signal above background for labeling durations as short as 12 hours
• Analysis revealed greater sensitivity for heat shock-induced proteins compared to global proteomics
• Gene set enrichment analysis confirmed significant enrichment of heat shock response proteins in BONCAT samples

Limitations

• Row count is unknown, which may limit suitability assessment
• Column-level documentation is absent; field semantics must be inferred after download
• Dataset is 927.2 KB, indicating a limited scope and likely small sample size

Provenance

Source

figshare

Collection Method

Proteins were enriched and identified via mass spectrometry after azidohomoalanine labeling in larval zebrafish.

Time Range

Labeling durations as short as 12 hours, but specific experimental dates are not provided.

Freshness

Last updated 2026-04-14 20:04:12; freshness should be verified

Geography

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