Zebrafish Proteomic Changes Using Bioorthogonal Noncanonical Amino Acid Tagging

A 1.0 MB dataset from figshare by Sophie E. Miller, last updated in April 2026. It contains mass spectrometry results from a time-resolved proteomic analysis in larval zebrafish using Bioorthogonal Noncanonical Amino Acid Tagging (BONCAT). The data was generated to identify proteins synthesized within defined time windows, including a proof-of-concept investigation of proteomic changes in response to heat shock.

Use Cases

• Identifying transiently synthesized proteins based on BONCAT enrichment data.
• Analyzing proteomic changes in response to stress, such as heat shock, based on the described experimental results.
• Comparing the sensitivity of BONCAT versus global proteomics for detecting time-dependent molecular responses.
• Investigating the roles of proteins in stress responses, lipid metabolism, and neural regulation based on the findings mentioned in the description.

Strengths

• Data is associated with a proof-of-concept experiment demonstrating significant signal above background for labeling durations as short as 12 hours.
• The described analysis revealed upregulated heat shock proteins with greater sensitivity than global proteomics.
• Gene set enrichment analysis confirmed significant enrichment of heat shock response proteins in BONCAT samples.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare

Collection Method

Proteins were enriched and identified via mass spectrometry after labeling larval zebrafish with azidohomoalanine.

Time Range

Labeling durations as short as 12 hours were used.

Freshness

Last updated 2026-04-14 20:04:11; freshness should be verified.

Geography

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