Zebrafish Time-Resolved Proteomic Data Using BONCAT Labeling

5.8 MB of proteomic data from larval zebrafish using Bioorthogonal Noncanonical Amino Acid Tagging (BONCAT) to identify proteins synthesized within defined time windows. The dataset, created by Sophie E. Miller and last updated in April 2026, includes results from labeling durations as short as 12 hours and a proof-of-concept investigation of proteomic changes in response to heat shock. Analysis revealed upregulated heat shock proteins and differentially expressed proteins involved in stress responses, lipid metabolism, and neural regulation.

Use Cases

• Training models to identify transient protein synthesis changes based on BONCAT labeling data.
• Analyzing the molecular underpinnings of heat shock response based on upregulated protein sets.
• Comparing sensitivity of targeted vs. global proteomics based on the described signal-to-background results.
• Investigating links between stress responses, lipid metabolism, and neural regulation based on the identified differentially expressed proteins.

Strengths

• Data is derived from a model organism (zebrafish) noted for its genetic tractability.
• Labeling was achieved in time windows as short as 12 hours, suggesting temporal precision.
• The method demonstrated greater sensitivity for heat shock proteins compared to global proteomics, as confirmed by gene set enrichment analysis.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for large-scale modeling.
• The dataset is small (5.8 MB), which may limit the complexity of analyses possible.

Provenance

Source

figshare

Collection Method

Proteins were labeled with azidohomoalanine, enriched, and identified via mass spectrometry.

Time Range

Labeling durations as short as 12 hours; specific experimental dates are not provided.

Freshness

Last updated 2026-04-14 20:04:09

Geography

Laboratory study; specific location is not provided.