Zebrafish Proteomic Changes During Heat Shock Using BONCAT

A 6.2 MB dataset from figshare, last updated April 14, 2026, by author Sophie E. Miller. It contains mass spectrometry results from a time-resolved proteomic analysis in larval zebrafish using Bioorthogonal Noncanonical Amino Acid Tagging (BONCAT). The data was generated to investigate protein synthesis changes, particularly in response to a heat shock stimulus, with labeling durations as short as 12 hours.

Use Cases

• Identifying transiently synthesized proteins based on the BONCAT enrichment method described.
• Analyzing proteomic responses to stress based on the heat shock experiment detailed.
• Comparing time-resolved protein synthesis to global proteomic profiles based on the sensitivity comparison mentioned.
• Investigating molecular pathways in stress response, lipid metabolism, and neural regulation based on the findings listed.

Strengths

• File size is precisely 6.2 MB.
• Data demonstrates significant signal above background for labeled proteins compared to unlabeled controls.
• Method showed greater sensitivity than global proteomics for detecting heat shock-induced proteins.
• Dataset is licensed under CC-BY-NC-4.0, specifying clear reuse terms.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare

Collection Method

Proteins were enriched and identified via mass spectrometry after labeling larval zebrafish with azidohomoalanine.

Time Range

Labeling durations as short as 12 hours were used.

Freshness

Last updated 2026-04-14 20:04:14; freshness should be verified.

Geography

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