Zebrafish Proteomic Changes During Heat Shock Using BONCAT Labeling

Sophie E. Miller's dataset, last updated April 14, 2026, provides proteomic analysis from larval zebrafish. It uses Bioorthogonal Noncanonical Amino Acid Tagging (BONCAT) to identify proteins synthesized within defined time windows as short as 12 hours. The data was generated as a proof of concept to investigate molecular responses to heat shock.

Use Cases

• Identify transiently synthesized proteins during stress responses based on BONCAT enrichment.
• Compare sensitivity of targeted vs. global proteomics based on the described heat shock analysis.
• Analyze changes in lipid metabolism and neural regulation pathways based on the identified differentially expressed proteins.
• Validate gene set enrichment results for specific biological processes based on the described methodology.

Strengths

• Data demonstrates significant signal above background for labeling durations as short as 12 hours.
• BONCAT analysis revealed greater sensitivity than global proteomics for detecting heat shock-induced proteins.
• File size is 2.2 MB, indicating a manageable download.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare, author Sophie E. Miller.

Collection Method

Proteins were labeled with azidohomoalanine, enriched, and identified via mass spectrometry.

Time Range

Labeling durations as short as 12 hours.

Freshness

Last updated 2026-04-14 20:04:08; freshness should be verified.

Geography

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