Zebrafish Proteomic Changes Using BONCAT Labeling and Heat Shock

A proteomic dataset from larval zebrafish using Bioorthogonal Noncanonical Amino Acid Tagging (BONCAT) to identify proteins synthesized within defined time windows. The data, published by Sophie E. Miller in 2026, includes results from labeling durations as short as 12 hours and a proof-of-concept investigation of proteomic changes in response to heat shock. It contains mass spectrometry-identified proteins, with comparisons to unlabeled controls and whole lysates.

Use Cases

• Analyzing transient protein synthesis changes in response to heat shock based on BONCAT-labeled protein data.
• Comparing the sensitivity of targeted versus global proteomics for detecting stress responses based on the described methodology.
• Investigating the role of proteins in lipid metabolism and neural regulation during stress based on the identified differentially expressed proteins.
• Validating gene set enrichment analysis methods for time-resolved proteomic datasets based on the described confirmation step.

Strengths

• Data demonstrates significant signal above background for labeling durations as short as 12 hours.
• BONCAT analysis revealed upregulated heat shock proteins with greater sensitivity than global proteomics, as confirmed by gene set enrichment analysis.
• Dataset is openly available under the CC-BY-NC-4.0 license.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is small in scale at 762.6 KB, indicating limited scope.

Provenance

Source

figshare

Collection Method

Proteins were enriched and identified via mass spectrometry after azidohomoalanine labeling in larval zebrafish.

Time Range

Labeling durations as short as 12 hours were used.

Freshness

Last updated 2026-04-14 20:04:05; freshness should be verified.

Geography

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