Time-Resolved Proteomic Analysis in Larval Zebrafish Using BONCAT

A proteomic dataset from larval zebrafish using Bioorthogonal Noncanonical Amino Acid Tagging (BONCAT) to identify proteins synthesized within defined time windows. The data includes results from labeling durations as short as 12 hours and a proof-of-concept investigation of proteomic changes in response to heat shock. The dataset was authored by Sophie E. Miller and last updated in April 2026.

Use Cases

• Analyze time-dependent protein synthesis based on BONCAT labeling durations mentioned in the description
• Investigate heat shock response mechanisms based on upregulated proteins identified via mass spectrometry
• Study transient molecular responses in stress, lipid metabolism, and neural regulation based on differentially expressed proteins highlighted in the description

Strengths

• Data demonstrates significant signal above background for labeling durations as short as 12 hours.
• Analysis revealed greater sensitivity for detecting heat shock-induced proteins compared to global proteomics.
• Gene set enrichment analysis confirmed significant enrichment of heat shock response proteins in BONCAT samples.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.

Provenance

Source

figshare

Collection Method

Proteins were enriched and identified via mass spectrometry after BONCAT labeling in larval zebrafish.

Time Range

Labeling durations as short as 12 hours.

Freshness

Last updated 2026-04-14 20:04:15.

Geography

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