S. cerevisiae Transcription Profiling Under Low-Shear Microgravity

A significant number of genes were up- or down-regulated by at least two fold in Saccharomyces cerevisiae cells grown for 5 or 25 generations in a High Aspect Ratio Vessel (HARV) to model microgravity. The study, conducted by NASA, compared four conditions with three replicates each, identifying genes involved in cell wall integrity, budding, and environmental stress response. The results provide clues to eukaryotic cell adaptation in microgravity, with potential implications for human spaceflight.

Use Cases

• Identify unique transcriptional responses to microgravity based on the 74% of genes not involved in the general environmental stress response.
• Analyze changes in cell wall integrity signaling pathways based on MAP kinase cascade genes mentioned in the description.
• Study phenotypic responses like budding and cell separation based on associated gene transcription changes.
• Compare genomic adaptation over time based on data from 5-generation and 25-generation growth periods.

Strengths

• Includes three biological replicates for each of the four experimental conditions.
• Identifies a specific proportion (26% vs 74%) of genes responding to general stress versus unique microgravity stress.
• Data originates from the authoritative source NASA.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data is stored in a BIN file format, which may require specialized tools for access.

Provenance

Source

National Aeronautics and Space Administration

Collection Method

Whole genome microarray hybridization of S. cerevisiae cultures grown in a rotating High Aspect Ratio Vessel (HARV) bioreactor.

Time Range

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Freshness

Last updated 2026-03-13 20:12:03.043816; freshness should be verified.

Geography

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