Transcriptional and proteomic response of Pseudomonas aeruginosa PAO1 to spaceflight condi

NASA research on the transcriptional and proteomic response of the opportunistic pathogen Pseudomonas aeruginosa PAO1 to low-Earth orbit conditions. The study compares spaceflight-grown cultures with ground controls, identifying differential regulation of 167 genes and 28 proteins. It highlights the role of the Hfq regulator and a shift to anaerobic growth, with implications for infectious disease risk in space.

Use Cases

• Modeling bacterial virulence adaptation based on differential gene expression data.
• Studying the role of the Hfq global regulator in bacterial stress responses.
• Comparing spaceflight-induced transcriptomic changes with ground-based microgravity analogues.
• Investigating anaerobic metabolic pathways like denitrification in low-fluid-shear environments.

Strengths

• Direct comparison of spaceflight and ground control samples in biological triplicate.
• Identifies 167 differentially regulated genes and 28 proteins, providing quantitative targets.
• First study to characterize global transcriptomic and proteomic response of Pseudomonas aeruginosa in the space habitat.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data format is listed as BIN, which may require specialized tools for access and analysis.

Provenance

Source

National Aeronautics and Space Administration

Collection Method

P. aeruginosa cultures were grown in space and compared with ground control samples; transcriptomic and proteomic profiles were analyzed.

Time Range

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Freshness

Last updated 2026-03-13 20:14:37.427227; freshness should be verified.

Geography

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