RR-23: Mouse Cerebellum Gene Expression After 38 Days in Space

January 2021 data from the Rodent Research-23 mission, profiling gene expression in mouse cerebellums after 38 days of microgravity on the ISS. The dataset features 9 samples from the Flight group, 9 from Habitat Ground Control, and 8 from Vivarium Ground Control, with RNA sequenced to a target of 60 million clusters per sample. It was generated by the National Aeronautics and Space Administration from tissues collected post-flight.

Use Cases

• Identify differential gene expression in the cerebellum based on spaceflight exposure versus ground control conditions.
• Analyze the impact of microgravity on vascular and lymphatic system genes relevant to vision, as mentioned in the study objective.
• Compare transcriptional profiles between Flight, Habitat Ground Control, and Vivarium Ground Control groups to isolate environmental effects.
• Validate RNA-seq findings using the technical replicates from independent library preparations included for one sample per group.

Strengths

• Includes a controlled experimental design with three distinct groups: Flight (n=9), Habitat Ground Control (n=9), and Vivarium Ground Control (n=8).
• RNA sequencing was performed to a specified depth target of 60 million clusters per sample with paired-end 150 bp reads.
• Contains a technical replicate for one sample in each group from an independent library preparation, aiding validation.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for specific analytical needs.

Provenance

Source

National Aeronautics and Space Administration

Collection Method

Experimental data from the Rodent Research-23 mission; mice were flown to the ISS on SpaceX-21, maintained for 38 days, returned, and tissues processed for RNA extraction and sequencing.

Time Range

January 2021

Freshness

Last updated 2026-03-13 19:53:48.677545; freshness should be verified.

Geography

International Space Station and terrestrial processing at Kennedy Space Center and Texas A&M University.