RR-10 Mission: Mouse Colon Gene Expression Under Spaceflight

39 bulk RNA sequencing samples from colon tissue of mice flown on the Rodent Research-10 mission to the International Space Station. The dataset includes gene expression data from 10 Basal, 9 Flight, 10 Ground, and 10 Vivarium control mice, collected by NASA. The flight mice were exposed to 28-29 days of microgravity.

Use Cases

• Identify differentially expressed genes in colon tissue based on exposure to microgravity.
• Compare gene expression profiles between wild-type and p21-null mouse strains under spaceflight conditions.
• Analyze the impact of simulated spaceflight environments (Ground control) versus actual spaceflight on transcriptional activity.
• Investigate the interaction between genetic background (strain) and environmental stressors (spaceflight, vivarium, ground habitat) on cellular mechanisms.

Strengths

• Includes data from 39 animals across four distinct experimental groups (Basal, Flight, Ground, Vivarium) for controlled comparison.
• Experimental design incorporates three ground control groups and a specific timeline of bone marker injections for precise biological context.
• Data originates from a controlled NASA mission (RR-10) with standardized sample processing and preservation protocols.

Limitations

• Row count and column-level documentation are unknown, which may limit suitability assessment.
• Description metadata is limited; actual data quality and structure require manual inspection after download.

Provenance

Source

National Aeronautics and Space Administration

Collection Method

Bulk RNA sequencing (ribodepleted) of colon tissue from mice flown on the SpaceX-21 mission to the ISS and corresponding ground controls.

Time Range

Data collection corresponds to the RR-10 mission timeline, with flight duration of 28-29 days.

Freshness

Last updated 2026-03-13 19:58:19.509342; freshness should be verified.

Geography

Samples from mice on the International Space Station and in ground-based simulators (ISSES).