RRRM-1: Liver RNA-Seq Data from Mice on the International Space Station

160 female BALB/cAnNTac mice were flown on the International Space Station for the Rodent Research Reference Mission-1. This dataset features ribodepleted total RNA-seq data from livers dissected from all groups, with 7-10 samples per group and ERCC spike-in controls. The data was generated by the National Aeronautics and Space Administration and last updated in March 2026.

Use Cases

• Identify gene expression changes due to spaceflight based on comparisons between flight and ground control groups.
• Analyze age-related differences in spaceflight response based on data from young (10-12 week) and old (32 week) mice.
• Study the effects of mission duration and recovery by comparing samples from on-orbit sacrifice (22-23 days) and live animal return (40 days flight + 2 days recovery).
• Normalize and calibrate RNA-seq data using the included ERCC spike-in controls (Mix 1 or Mix 2).

Strengths

• Includes data from 160 mice across multiple control and experimental groups, providing a robust experimental design.
• Uses ribodepleted total RNA-seq with ERCC spike-in controls, which suggests a focus on accurate transcript quantification.
• Directly compares spaceflight effects with independent ground, basal, and vivarium controls.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for specific analytical needs.
• The data format is listed as BIN, which may require specialized tools or knowledge to process.

Provenance

Source

National Aeronautics and Space Administration

Collection Method

Ribodepleted total RNA sequencing of liver tissue dissected from mice flown on the International Space Station.

Freshness

Last updated 2026-03-13 19:43:54.725687; freshness should be verified.

Geography

International Space Station and associated ground control facilities.