RR-23 Mission: Mouse Lung Transcriptional Profiling After 38 Days in Space

NASA's RR-23 mission dataset contains transcriptional profiling data from the lungs of mice exposed to microgravity on the International Space Station for 38 days. The data includes RNA sequencing results from 8 Flight, 9 Vivarium Ground Control, and 6 Habitat Ground Control mouse samples collected in January 2021. Libraries were sequenced with a target of 60 million clusters per sample using 150 base pair paired-end reads.

Use Cases

• Identify gene expression changes in lung tissue based on exposure to spaceflight conditions.
• Compare transcriptomic profiles between flight, habitat control, and vivarium control groups mentioned in the description.
• Investigate the biological impact of 38 days of microgravity on a mouse model system.
• Analyze RNA sequencing data generated with a target depth of 60 million clusters per sample.

Strengths

• Data originates from a controlled NASA spaceflight experiment with defined Flight, Habitat Ground Control, and Vivarium Ground Control groups.
• Sequencing was performed with a specified target depth of 60 million clusters per sample using 150bp paired-end reads.
• Experimental timeline and procedures, including a 38-day microgravity exposure and post-flight processing dates, are documented.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for specific analyses.
• The dataset description does not specify the exact number of features or variables measured.

Provenance

Source

National Aeronautics and Space Administration

Collection Method

RNA extracted from flash-frozen mouse lung tissue after spaceflight, followed by library generation and sequencing.

Time Range

Sample collection occurred in January 2021.

Freshness

Last updated 2026-03-13 19:50:28.300781; freshness should be verified.

Geography

Samples were collected from mice flown on the ISS and processed at Texas A&M University, USA.