RRRM-1: Mouse Mammary Gland RNA-Seq Data from Spaceflight Mission

160 female BALB/cAnNTac mice were flown on the International Space Station's RRRM-1 mission to study age and spaceflight effects. This dataset features ribodepleted total RNA-seq data from mammary glands of six Live Animal Return groups, with 5-10 livers per group and ERCC spike-in controls. Data was generated by the National Aeronautics and Space Administration, with a target sequencing depth of 60 million clusters at paired-end 150 bp.

Use Cases

• Identify gene expression changes in mammary tissue based on spaceflight exposure mentioned in the description
• Compare transcriptional profiles based on age differences (young vs. old mice) described in the experimental design
• Assess recovery effects after return to Earth based on the Live Animal Return (LAR) group data
• Control for technical variation using the ERCC spike-in controls included in all samples

Strengths

• Includes data from 160 mice across multiple control groups (ground, basal, vivarium) for robust comparison
• Experimental design controls for age with 10-12 week-old and 32 week-old cohorts
• Sequencing targeted a depth of 60 million clusters at paired-end 150 bp

Limitations

• Column-level documentation is absent; field semantics must be inferred after download
• Row count is unknown, which may limit suitability assessment

Provenance

Source

National Aeronautics and Space Administration

Collection Method

Ribodepleted total RNA-seq from dissected mammary glands and livers of mice flown on the International Space Station.

Time Range

Data from the Rodent Research Reference Mission-1 (RRRM-1/RR-8).

Freshness

Last updated 2026-03 13 19:49:50.922487; freshness should be verified

Geography

Samples from mice flown on the International Space Station, with ground controls.