Brachypodium distachyon Seedling RNA-seq from ISS Veggie Experiment

Ribodepleted RNA-seq data from roots and shoots of three Brachypodium distachyon accessions grown on the International Space Station. Seedlings were grown in Veggie hardware for 5 days under controlled light conditions before harvest. The dataset was produced by the National Aeronautics and Space Administration and last updated in March 2026.

Use Cases

• Identify gene expression changes in monocot plants under microgravity based on the RNA-seq data.
• Compare transcriptional responses between different Brachypodium distachyon accessions (Bd21, Bd21-3, Gaz8) grown in space.
• Investigate differential root and shoot adaptation to spaceflight based on the tissue-specific samples.
• Validate findings from dicot model plant studies (e.g., Arabidopsis) in a monocot model system.

Strengths

• Data is from a controlled experiment on the International Space Station using the Veggie hardware.
• Includes three distinct genetic accessions (Bd21, Bd21-3, Gaz8) of the model grass Brachypodium distachyon.
• Samples are from dissected roots and shoots, allowing for tissue-specific analysis.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data is stored in BIN format, which may require specific tools for access and analysis.

Provenance

Source

National Aeronautics and Space Administration

Collection Method

Seedlings were grown on the ISS in Veggie hardware under a 24-hour photoperiod for 5 days, then harvested and preserved in RNAlater for RNA-seq.

Freshness

Last updated 2026-03-13 19:44:14.575379; freshness should be verified.

Geography

International Space Station