RR-23: Mouse Spleen Transcriptional Profiles After 38 Days in Space

Twenty male C57BL/6J mice were flown to the ISS for 38 days on SpaceX-21 to study spaceflight's effects on vision. Post-flight, spleen tissue from 9 Flight, 10 Habitat Ground Control, and 9 Vivarium Ground Control mice was preserved for RNA sequencing. The National Aeronautics and Space Administration generated this dataset, with samples processed in January 2021.

Use Cases

• Identify gene expression changes in spleen tissue based on exposure to microgravity.
• Compare transcriptional profiles based on the three experimental groups: Flight, Habitat Ground Control, and Vivarium Ground Control.
• Investigate immune system adaptations based on the 38-day spaceflight duration.
• Analyze RNA sequencing data based on the target of 60 million clusters per sample with paired-end 150 bp reads.

Strengths

• Includes a controlled experimental design with three distinct groups (Flight, HGC, VGC) totaling 28 samples.
• Specifies precise experimental parameters: 38-day spaceflight duration, mouse strain (C57BL/6J), and RNA sequencing target depth.
• Managed by a authoritative source, the National Aeronautics and Space Administration.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data may reflect temporal and experimental bias inherent to a single, highly specific mission.

Provenance

Source

National Aeronautics and Space Administration

Collection Method

Experimental data from the Rodent Research-23 (RR-23) mission; spleen tissue preserved in RNAlater, RNA extracted, and libraries sequenced.

Time Range

Spaceflight mission occurred in 2020-2021, with tissue collection and processing in January 2021.

Freshness

Last updated 2026-03-13 19:52:32.019653; freshness should be verified.

Geography

Samples originated from mice flown on the International Space Station and processed at Texas A&M University, USA.